Understanding the Effects of Lactose Hydrolysis Modeling on the Main Oligosaccharides in Goat Milk Whey Permeate.

Enzymatic hydrolysis of lactose is a crucial step to improve the efficiency and selectivity of membrane-based separations toward the recovery of milk oligosaccharides free from simple sugars. Response surface methodology was used to investigate the effects temperature (25.9 to 54.1 °C) and amount of enzyme (0.17 to 0.32% w/w) at 1, 2, and 4 h of reaction on the efficiency of lactose hydrolysis by Aspergillus oryzae β-galactosidase, preservation of major goat whey oligosaccharides, and on the de-novo formation of oligosaccharides. Lactose hydrolysis above 99% was achieved at 1, 2, and 4 h, not being significantly affected by temperature and amount of enzyme within the tested conditions. Formation of 4 Hexose (Hex) and 4 Hex 1 Hex and an increased de-novo formation of 2 Hex 1 N-Acetyl-Neuraminic Acid (NeuAc) and 2 Hex 1 N-Glycolylneuraminic acid (NeuGc) was observed in all treatments. Overall, processing conditions using temperatures ≤40 °C and enzyme concentration ≤0.25% resulted in higher preservation/formation of goat whey oligosaccharides

Treatment of estrogen-induced dermatitis with omalizumab

In 1945, Drs Bernhard Zondek and Yehuda Bromberg demonstrated intradermal treatment with estrone and estradiol benzoate induced urticarial lesions in some patients.1 Fifty years later, Shelley et al,2 who introduced the concept of progesterone dermatitis several decades prior, defined estrogen dermatitis based on studies of 7 women with premenstrual flares of skin eruptions including papulovesicular, urticarial, or eczematous lesions or generalized pruritus. Previously described therapies for estrogen dermatitis include estrogen desensitization, tamoxifen, leuprolide, and oophorectomy.3 Here we report a case of estrogen-induced dermatitis successfully treated with omalizumab

Uplift and Seismicity driven by Magmatic Inflation at Sierra Negra Volcano, Galápagos Islands

Catalogue of detected earthquakes and cGPS uplift timeseries for Sierra Negra Volcano, Galapagos Island

Perceptual oscillations in gender classification of faces, contingent on stimulus history

Perception is a proactive ‘‘predictive’’ process, in which the brain takes advantage of past experience to make informed guesses about the world to test against sensory data. Here we demonstrate that in the judgment of the gender of faces, beta rhythms play an important role in communicating perceptual experience. Observers classified in forced choice as male or female, a sequence of face stimuli, which were physically constructed to be male or female or androgynous (equal morph). Classification of the androgynous stimuli oscillated rhythmically between male and female, following a complex waveform comprising 13.5 and 17 Hz. Parsing the trials based on the preceding stimulus showed that responses to androgynous stimuli preceded by male stimuli oscillated reliably at 17 Hz, whereas those preceded by female stimuli oscillated at 13.5 Hz. These results suggest that perceptual priors for face perception from recent perceptual memory are communicated through frequency-coded beta rhythms

Prevalence and experiences of food insecurity among immigrant women connected to perinatal programs at a community-based organization in Edmonton, Canada

Purpose: The purpose of this paper is to investigate the prevalence of household food insecurity among immigrant women connected to perinatal programs offered through a community-based organization in Edmonton, and to explore their experiences in coping with food insecurity. Design/methodology/approach: This study utilized a mixed methods research design. A community-based participatory research approach was used to engage health workers who were connected to immigrant women and families through the Multicultural Health Brokers Cooperative in Edmonton. Through the health workers a sample of 213 immigrant women connected to their perinatal programs completed the Household Food Security Survey. Following the survey, 17 women completed semi-structured interviews which were analyzed using content analysis. Findings: The vast majority of women (94 percent (n=199)) lived in food insecure households, and 53 percent (n=112) in severely food insecure. In semi-structured interviews, women specifically described not having enough money to buy vegetables, fruit and meat, and perceiving a lack of control over foods they ate and offered to their families. Practical implications: This study highlights the need for support to be provided to immigrant families for acquiring healthy food in Canada. Originality/value: The mixed methods design with a decent sample of often underrepresented research participants highlights an area in need of further research and greater support

Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth

<p>Abstract</p> <p>Background</p> <p>Chemo/radio-resistance is a major obstacle in treating advanced ovarian cancer. The efficacy of current treatments may be improved by increasing the sensitivity of cancer cells to chemo/radiation therapies. Curcumin is a naturally occurring compound with anti-cancer activity in multiple cancers; however, its chemo/radio-sensitizing potential is not well studied in ovarian cancer. Herein, we demonstrate the effectiveness of a curcumin pre-treatment strategy for chemo/radio-sensitizing cisplatin resistant ovarian cancer cells. To improve the efficacy and specificity of curcumin induced chemo/radio sensitization, we developed a curcumin nanoparticle formulation conjugated with a monoclonal antibody specific for cancer cells.</p> <p>Methods</p> <p>Cisplatin resistant A2780CP ovarian cancer cells were pre-treated with curcumin followed by exposure to cisplatin or radiation and the effect on cell growth was determined by MTS and colony formation assays. The effect of curcumin pre-treatment on the expression of apoptosis related proteins and β-catenin was determined by Western blotting or Flow Cytometry. A luciferase reporter assay was used to determine the effect of curcumin on β-catenin transcription activity. The poly(lactic acid-<it>co</it>-glycolic acid) (PLGA) nanoparticle formulation of curcumin (Nano-CUR) was developed by a modified nano-precipitation method and physico-chemical characterization was performed by transmission electron microscopy and dynamic light scattering methods.</p> <p>Results</p> <p>Curcumin pre-treatment considerably reduced the dose of cisplatin and radiation required to inhibit the growth of cisplatin resistant ovarian cancer cells. During the 6 hr pre-treatment, curcumin down regulated the expression of Bcl-X_Land Mcl-1 pro-survival proteins. Curcumin pre-treatment followed by exposure to low doses of cisplatin increased apoptosis as indicated by annexin V staining and cleavage of caspase 9 and PARP. Additionally, curcumin pre-treatment lowered β-catenin expression and transcriptional activity. Nano-CUR was successfully generated and physico-chemical characterization of Nano-CUR indicated an average particle size of ~70 nm, steady and prolonged release of curcumin, antibody conjugation capability and effective inhibition of ovarian cancer cell growth.</p> <p>Conclusion</p> <p>Curcumin pre-treatment enhances chemo/radio-sensitization in A2780CP ovarian cancer cells through multiple molecular mechanisms. Therefore, curcumin pre-treatment may effectively improve ovarian cancer therapeutics. A targeted PLGA nanoparticle formulation of curcumin is feasible and may improve the <it>in vivo </it>therapeutic efficacy of curcumin.</p

Extracellular electrophysiological measurements of cooperative signals in astrocytes populations

Astrocytes are neuroglial cells that exhibit functional electrical properties sensitive to neuronal activity and capable of modulating neurotransmission. Thus, electrophysiological recordings of astroglial activity are very attractive to study the dynamics of glial signaling. This contribution reports on the use of ultra-sensitive planar electrodes combined with low noise and low frequency amplifiers that enable the detection of extracellular signals produced by primary cultures of astrocytes isolated from mouse cerebral cortex. Recorded activity is characterized by spontaneous bursts comprised of discrete signals with pronounced changes on the signal rate and amplitude. Weak and sporadic signals become synchronized and evolve with time to higher amplitude signals with a quasi-periodic behavior, revealing a cooperative signaling process. The methodology presented herewith enables the study of ionic fluctuations of population of cells, complementing the single cells observation by calcium imaging as well as by patch-clamp techniques.Portuguese Foundation for Science and Technology (FCT) [PTDC/EEI-AUT/5442/2014]; Instituto de Telecomunicacoes [UID/Multi/04326/2013]; Associated Laboratory - Institute of Nanoscience and Nanotechnology [POCI-01-0145-FEDER-016623]; [PTDC/CTM-NAN/3146/2014]info:eu-repo/semantics/publishedVersio

Lessons on Collecting Data from Autistic Children Using Wrist-Worn Sensors

Autism is a diverse neurodevelopmental condition that has a hugely varying impact of the lives of autistic people. It is only in the last decades that a greater understanding and public awareness of the autism spectrum has come about, in-part thanks to a growing body of research into the condition. Wearable technology offers great promise in furthering autism research by providing an ability to do detailed behavioral analysis in real-life settings, such as in schools, with minimal intrusion. Such work is particularly crucial in exploring behaviours of those with complex needs and intellectual disabilities, a group who traditionally have been under-served. To achieve this there is a need for wearables that are both practical and acceptable to the individuals being studied. This paper presents our findings from a human-centred design approach to developing and deploying wrist-worn sensors among a diverse population of 16 autistic and 12 neurotypical children over a period of several months. Findings and recommendations from this work highlight the need to take both sensory factors and emotional dysregulation into account when designing wearables for autism. Individual aesthetic and social considerations are particularly important for older children. Equally, a period of sensor desensitisation is necessary when working among those with more complex needs